Document Details
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Document Type |
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Thesis |
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Document Title |
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Microbial and Molecular Identification of Microbiota in Baby Intensive Care Unit at King Abdulaziz University Hospital التعريف الميكروبي والجزيئي للميكروبات في وحدة العناية المركزة للأطفال بمستشفى جامعة الملك عبدالعزيز |
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Subject |
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Faculty of Arts and Humanities |
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Document Language |
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Arabic |
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Abstract |
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New-born babies in neonatal intensive care units (NICUs) are usually immunocompromised. So, healthcare-associated infections (HAIs) are a serious problem for babies in NICUs. The healthcare environment and healthcare workers hands can be a source of transmission. Therefore, there is a need to determine the microbial sources, isolate and identify the contaminants to improve disinfection protocols followed and decrease HAIs. 122 bacterial strains were isolated from King Abdulaziz University Hospital, Neonatal intensive care unit (NICU) on nutrient agar (NA) medium. The samples were collected from surfaces, equipment and nurses’ hands from three zones divided depending on close to and distant from the patient. The tested inanimate environments included incubators, oxygen monitors, heart monitors, temperature monitors, intravenous (IV), stethoscopes, tables, IV trolleys, weighing scales, windows, and sinks. The results showed that all tested equipment and surrounding surfaces of the unit were contaminated with various distribution of bacteria except sinks. Out of 122, )78 (of bacteria isolates were cocci and )44(were bacilli. Also, )105(isolates were Gram-positive and )17(were Gram-negative. Using 16S rRNA gene sequencing techniques, 11 genera were isolated, namely, Enterococcus, Staphylococcus, Bacillus, Pseudomonas, Micrococcus, Escherichia, Enterobacter, Klebsiella, Citrobacter, lelliottia, and Acinetobacter. However, most isolated bacteria were Staphylococcus epidermidis followed by Bacillus cereus. The susceptibility tests were performed by Vitek 2 with cards (AST-GP67, AST-N292, AST-N291), and the results indicated pathogenic bacterial strains responded differently to various tested antibiotics; however, some of these strains were found resistant against several antibiotics. Overall, the present study indicated the presence of some bacteria species which may possess threats to the health of new-born patients, and therefore, it is important to improve the disinfection protocols and investigate the unit regularly for potential microbial contamination to ensure the human safety. |
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Supervisor |
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Dr. Ahmed Mahmoud Al-Hejin |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Co-Supervisor |
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Prof. Rukaia Mohmammed Gashgari |
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Added Date |
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Tuesday, May 19, 2020 |
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Researchers
| رقية محمد الجابري | AL-Jabri, Roqaiah Mohammed | Researcher | Master | |
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